Login / Register

Username:


Password: [Lost?]



New User? Click here for your FREE subscription



Laboratory Professionals
Laboratory Professionals Laboratory Professionals

Follow Us


NEWS-Line on Twitter NEWS-Line on Facebook NEWS-Line on Google+ NEWS-Line on LinkedIn NEWS-Line on Pinterest


Laboratory Conferences &
Educational Opportunities



Sept. 9 - 11

ANNUAL MEETING

American Society for Clinical Pathology

More Events

Paper-based test could diagnose Lyme disease at early stages | NEWS-Line for Laboratory Professionals
 


Paper-based test could diagnose Lyme disease at early stages


Source:

After a day hiking in the forest, the last thing a person wants to discover is a tick burrowing into their skin. Days after plucking off the bloodsucking insect, the hiker might develop a rash resembling a bull’s-eye, a tell-tale sign of Lyme disease. Yet not everybody who contracts Lyme disease gets the rash. Now, researchers reporting in ACS Nano have devised a blood test that quickly and sensitively diagnoses the disease at early stages.

About 300,000 cases of Lyme disease, which is caused by the tick-borne bacteria Borrelia burgdorferi, are diagnosed in the U.S. each year, according to the U.S. Centers for Disease Control and Prevention. Early symptoms of the disease include the characteristic skin rash, along with fever, headache, chills and muscle aches. If not treated promptly with antibiotics, more severe symptoms, such as facial palsy, nerve pain, heart palpitations and arthritis, can occur. However, 10–20% of infected people do not develop the rash, and existing diagnostic blood tests are slow, costly or insensitive at early stages, when treatment is most effective. Aydogan Ozcan and colleagues wanted to develop a fast, easy-to-use and inexpensive blood test to diagnose Lyme disease soon after infection.

The researchers built a handheld, paper-based device to detect antibodies against the B. burgdorferi bacteria in serum samples. The device included a sensing membrane that contained several spots covering seven bacterial antigens and a synthetic peptide. Antibodies from serum samples that attached to the spots were detected with a solution that changed color, depending on the amount of antibody captured. The researchers took pictures of the color changes on a smart phone, then analyzed all of the spots with a neural network they developed that could determine whether the sample was positive or negative for Lyme disease. When tested on 50 blood samples from people with or without early-stage Lyme disease, the assay had a specificity of 96.3% and a sensitivity of 85.7%. In addition to being much more sensitive than existing tests, the assay requires 15 minutes to complete and costs only 42 cents per test.

The authors acknowledge funding from the Steven and Alexandra Cohen Foundation.

Full article: https://pubs.acs.org/doi/full/10.1021/acsnano.9b08151

CONTACT:
Aydogan Ozcan, Ph.D.
Department of Electrical & Computer Engineering
University of California, Los Angeles
Los Angeles, California 90025
Phone: 310-825-0915
Email: [email protected]

Source: ACS Nano

Photo: ACS Nano






Share This!


Laboratory Jobs



Medical Technologist OR Medical Laboratory Technologist **Sign on Bonuses are available for specific positions*

St Lukes Hospital
Chesterfield & Des Peres in St. Louis, Missouri

Medical Technologists

Enzo Clinical Labs
Farmingdale, New York

Medical Laboratory Scientist

Baptist Health
Alabama

MT or MLT

American Pathology Partners|UniPath
Durango, Colorado

Med Tech

The Blood Center
Louisiana

Clinical/Medical Technologist 1

Washington State University - Veterinary Teaching Hospital Clinical Pathology Laboratory
Pullman, Washington

More Jobs
(Dismiss) Thank you for visiting NEWS-Line! Please sign up, login, or follow us on your favorite social networks
to receive custom tailored eNews, job listings, and educational opportunities for your specific profession.